Discoordination of ribosomal RNA metabolism during metabolic shifts of Spirodela plants

Abstract

The effects of metabolic shifts on nucleic acid syntheses have been widely studied in prokaryotes, but not in plants because of a paucity of suitable systems. Spirodela (Duckweed) was thus used to ascertain the response of the nucleocytoplasmic (nc) and plastid ribosomal RNA metabolisms to partial and total carbon deprivation. The 0.56 × 10 6 M m plastid rRNA is the one species of RNA most affected by metabolic shifts; unlike other species, its appearance is delayed by deprivation and it appears more rapidly than other species on transfer from dark to light. The data suggest a discoordination between the transcription and processing of plastid ribosomal precursors. Incorporation into all nc and plastid rRNAs was severely reduced and all rRNA precursors accumulated in green plants that were completely deprived of carbon by transferring to the dark, without sucrose. The amounts of nc and plastid precursors transcribed readjusted to the reduced amounts processed to mature RNA only after long periods in the dark with sucrose. This delay involved the formation of new colorless plants. Less plastid RNAs, compared to nc RNAs are found in the dark steady state.