Discontinuous gas exchange patterns of beet armyworm pupae, Spodoptera exigua (Lepidoptera: Noctuidae): effects of Bacillus thuringiensis Cry1C toxin, pupal age and temperature

Abstract

Abstract. Changes in the discontinuous gas exchange cycle of pupal beet armyworm, Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae), exposed or not to Cry1C Bacillus thuringiensis toxin, are examined against developmental age (1–7 days) and at different temperatures (10–25 °C) using flow through respirometry. Both exposed and nonexposed pupae exhibit discontinuous gas exchange, but only at 10 °C; the frequency of cyclic release of CO2 increases with increasing temperatures. The three phases of the discontinuous gas exchange cycle are distinct for both treatment groups. However, the duration of each phase is significantly greater for pupae exposed previously to toxin. The closed phase is 40 ± 14% longer, the flutter phase 23 ± 19% longer, and the open phase is 28 ± 12% longer when pupae were exposed to toxin. Respiratory water loss is 4.5 ± 1.3% for toxin exposed pupae and 2.1 ± 2.4% for unexposed pupae. Furthermore, the exposed pupae have significantly greater cuticular permeability (26.01 ± 1.9 µg cm−2 h−1 mmHg−1) than the nonexposed pupae (9.64 ± 0.9 µg cm−2 h−1 mmHg−1). However, in both strains, cuticular transpiration (>93%) far exceeds respiratory transpiration. Overall, total water loss is significantly greater in pupae whose larvae are exposed to toxin compared with pupae from nontreated larvae. Toxin exposed pupae have a mean cycle duration of 60 ± 2.5 min whereas that of nonexposed pupae is 42 ± 1.8 min.(ml g−1 h−1) of the open phase is greater earlier in pupal life followed by a minimum at mid‐pupal stage and an increase at late‐pupal development in both treatment groups. Combining all 7 days, closed, flutter and open phase (ml g−1 h−1), pupae exposed to toxin produce significantly more CO2 during each phase. On average, toxin exposed pupae produce 52 ± 12, 43 ± 10 and 15 ± 37% more CO2 than the untreated pupae during the closed, flutter and open phases, respectively. Therefore, the present study reinforces the need to use insects of similar developmental age in studies of insect respiration patterns and energy metabolism.