Abstract
1. 1. Esterase A4 (Ease A4), a silkworm ( Bombyx mori) molecular timer, was incubated at various temperatures to study the mechanism of diapause-break by winter cold. 2. 2. The activity of the enzyme was very low during the early period, but it suddenly increased at a certain time of the incubation, depending upon the incubation temperature, and was followed by a rapid fall. 3. 3. Arrhenius plots of the velocities up to the peak of the transitory burst gave two straight lines. 4. 4. The cold induced a dissociation of a complex of Ease A4 with time-zero substance(s).
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