Abstract
Messenger RNP particles (mRNPs) move randomly within nucleoplasm before they exit from the nucleus through nuclear pore complexes. To further understand mRNP trafficking, we have studied the movement of a specific mRNP, the BR2 mRNP, in the nuclei of salivary gland cells in Chironomus tentans larvae. These polytene nuclei harbor giant chromosomes separated by vast regions of nucleoplasm, which allows to study mRNP mobility without interference of chromatin. The particles were fluorescently labeled by hybridization with microinjected RNA or DNA oligonucleotides, and their movement was examined by single particle tracking.
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