Discerning the effects of photoinhibition and photoprotection on the rate of oxygen evolution in Arabidopsis leaves

Abstract

Higher plants possess a set of interconnected processes to regulate light harvesting. Non-photochemical quenching of chlorophyll a fluorescence (NPQ) is the fastest process activated to protect the photosystem (PS) II from the absorption of excess light energy. However, damage of PSII reaction centers (RCIIs) is often inevitable, a phenomenon known as photoinhibition. Both NPQ and photoinhibition undermine PSII quantum yield (ΦPSII). Recently, we devised a fluorescence-based methodology that uses the coefficient of photochemical quenching measured in the dark following illumination (qPd) to assess the intactness of RCIIs. This procedure enables to express ΦPSII as a function (ƒ) of NPQ and qPd, ΦPSII=ƒ(NPQ,qPd), thus allowing to efficiently discern between the effects of protective NPQ and photoinhibition upon the efficiency of electron transport. In this study, we addressed the relationship between qPd and ΦPSII measured by photosynthetic oxygen evolution in intact leaves of Arabidopsis. We found a linear correlation between qPd and ΦPSII of oxygen evolution (as well as Fv/Fm). This relates to the fact that qPd reflects the onset of photoinhibition. These results further demonstrate the validity of the qPd parameter and underlying theory in quantitatively assessing PSII efficiency solely by using this effective and simple fluorescence technique.