Abstract
Disc electrophoresis is an effective method for the separation of the protein and protein-carbohydrate components of human salivary gland secretions. When submaxillary saliva is concentrated twenty times, 25 μl of the concentrate can be separated into fifteen components by electrophoresis for 30–50 minutes. Continuation for periods up to 2 hours further separates the slowly moving protein-carbohydrate complexes so that a total of eighteen components is separated. Reversal of the electrodes in other instances reveals an additional three components migrating to the cathode in pH 8.3 buffer. Since the amount of nondialyzable solids in the submaxillary saliva is around 200 mg%, the twenty-one components are separated from about 1 mg of solid material. Blood group substance A and glycoproteins of various sialic acid/fucose ratios are separated from other salivary proteins by electrophoresis. The method is also useful for the study of human parotid saliva and mixed human salivary secretions.
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