Disassociation of Vitamin D\u2019s Calcemic Activity and Non-calcemic Genomic Activity and Individual Responsiveness: A Randomized Controlled Double-Blind Clinical Trial

Arash Shirvani,Tyler Arek Kalajian,Michael F Holick,Anjeli Song

doi:10.1038/s41598-019-53864-1

Arash Shirvani, Tyler Arek Kalajian + Show 2 more

Open Access

https://doi.org/10.1038/s41598-019-53864-1

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Journal: Scientific Reports	Publication Date: Nov 27, 2019
Citations: 76	License type: open-access

Affiliation: Boston University

Abstract

The aims of this randomized controlled double-blind clinical trial were to assess the impact of vitamin D supplementation on calcium metabolism and non-calcemic broad gene expression by relating them to the individual’s responsiveness to varying doses of vitamin D3. Thirty healthy adults were randomized to receive 600, 4,000 or 10,000 IU/d of vitamin D3 for 6 months. Circulating parathyroid hormone (PTH), 25(OH)D, calcium and peripheral white blood cells broad gene expression were evaluated. We observed a dose-dependent increase in 25(OH)D concentrations, decreased PTH and no change in serum calcium. A plateau in PTH levels was achieved at 16 weeks in the 4000 and 10,000 IU/d groups. There was a dose-dependent 25(OH)D alteration in broad gene expression with 162, 320 and 1289 genes up- or down-regulated in their white blood cells, respectively. Our results clearly indicated that there is an individual’s responsiveness on broad gene expression to varying doses of vitamin D3. Vitamin D3 supplementation at 10,000 IU/d produced genomic alterations several fold higher than 4,000 IU/d even without further changes in PTH levels. Our findings may help explain why there are some inconsistency in the results of different vitamin D’s clinical trials.

Highlights

Www.nature.com/scientificreports cancer than placebo[13]
The current clinical trial was designed to evaluate the responsiveness of different doses of vitamin D supplementation (600 IU/d, 4,000 IU/d and 10,000 IU/d) on serum calcium, parathyroid hormone (PTH) and broad gene expression in white blood cells
This broad gene expression study describes the first insight into the genome wide regulation activity of different doses of vitamin D3 supplementation in human white blood cells

Summary

Introduction

Www.nature.com/scientificreports cancer than placebo[13]. Despite that vitamin D supplementation did not associate with a lower occurrence of total deaths from cancer than placebo, yet found that mortality due to cancer was significantly lower with vitamin D supplementation[13]. The current clinical trial was designed to evaluate the responsiveness of different doses of vitamin D supplementation (600 IU/d, 4,000 IU/d and 10,000 IU/d) on serum calcium, PTH and broad gene expression in white blood cells.

Results

Conclusion