Directional transfer of the genic multiple allele inherited male sterile line in Chinese cabbage

Abstract

A “directional transfer program” was designed and carried out according to the genotype on the male sterility locus of the target line, in order to improve the utilization of the multiple-allele inherited male sterile gene in Chinese cabbage and solve the problems in the transfer process of this gene. In the program, recurrent backcrossing was employed to transfer botanical traits, while the genotype was identified through test cross. In addition, we began to develop the male sterile line after the transfer of botanical traits was finished. Following the procedure, the male sterile gene was successfully transferred from “3A”, a white leafstalk inbred line in Chinese cabbage, to “206”, the target green leafstalk inbred line in cylindrical ecotype. The “GMS104”, a male sterile line with similar botanical traits to “206” with 100% male sterility and 100% male sterile plants, was bred. With “GMS104” as the female parent, an excellent hybridized combination “GMS104×B4” was finally obtained and it passed the variety certification and registration of minor crops in Liaoning, China. Based on the results, it was certified that male sterility and the other botanical and economic traits could be transferred to a target line simultaneously following the “directional transfer program” designed in our study. Key words: Chinese cabbage, genetic male sterile line, transfer.