Abstract
Multiform glioblastoma is the most common primary, highly invasive, malignant tumor of the central nervous system, with an extremely poor prognosis. The median survival of patients following surgical resection, radiation therapy and chemotherapy does not exceed 12–15 months and thus, novel approaches for the treatment of the disease are required. The phenomenon of the directed migration of stem cells in tumor tissue presents a novel approach for the development of technologies that facilitate the targeted delivery of drugs and other therapeutic agents to the tumor foci. Hematopoietic cluster of differentiation (CD)34+/CD133+ stem cells possess significant reparative potential and are inert with respect to normal neural tissue. The aim of the present study was to investigate the substantiation ability of adult hematopoietic progenitors to the directed migration of glioma cells. A C6 glioma cell line, a culture of hematopoietic CD34+/CD133+ stem cells and primary cultures of rat astrocytes and fibroblasts were used. The cells were co-cultured for five days. The results revealed the formation of cell shaft hematopoietic stem cells on the perimeter of the culture inserts containing the glioma culture. However, this was not observed in the wells with fibroblast and astrocyte cultures. The results indicated that hematopoietic stem cells exhibit a high potential for the directional migration of C6 glioma cells, which allows them to be considered as a promising cell line for the development of novel anticancer biomedical technologies and increases our understanding with regard to previously unclear aspects of glial tumor carcinogenesis.
Highlights
Multiform glioblastoma is the most common primary, highly invasive, malignant tumor of the central nervous system, with an extremely poor prognosis
We believe that hematopoietic CD34+/CD133+ stem cells may represent an alternative, as their collection and maintenance presents little difficulty, their use is not associated with ethical and legal restrictions and they have been successfully used for the treatment of cancer patients for >50 years [13]
C6 glioma cell culture presented a heterogeneous population of actively proliferating cells of different shapes and sizes (Fig. 2C)
Summary
Multiform glioblastoma is the most common primary, highly invasive, malignant tumor of the central nervous system, with an extremely poor prognosis. The phenomenon of the directional migration of neural stem and progenitor cells in tumor tissue has been a focus, and has presented a significant opportunity for the targeted delivery of drug molecules to tumor‐specific genes, antibodies and other therapeutic agents [5‐9]. In this way, the problem of selecting the optimal cell lines for transplantation is important as the use of the patients own neural stem cells carries a high risk of neoplastic transformation [10‐12]. We believe that hematopoietic CD34+/CD133+ stem cells may represent an alternative, as their collection and maintenance presents little difficulty, their use is not associated with ethical and legal restrictions and they have been successfully used for the treatment of cancer patients for >50 years [13]
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