Abstract

The selectivity of the bacterial porin OmpF from E. coli to small inorganic ions has been investigated by single channel experiments. In a recent study, we showed that the OmpF channel may function as a pH-regulated, biological, nanofluidic diode (J. Phys. Chem. B 110 (2006) 21205). Here we show that Reversal potential measurements done under asymmetric conditions of pH and salt concentration provide valuable information about the channel fixed charge distribution that cannot be extracted from the rectification displayed in current-voltage curves.

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