Abstract
An F- thr-ara ‡ ‡ Abbreviations used: lac, ara, gal, utilization of lactose, arabinose, galactose; his, pyr, pur, arg, thr, leu, pro, trp requirement for histidine, pyrimidine, purine, arginine, threonine, leucine, proline, tryptophan; T1 r, T1,5 r, resistance to phage T1, phages T1 and T5; att80, chromosome attachment site for φ80; Sm s, Sm r, sensitive and resistant to streptomycin; φ80d ara, φ80d lac, defective φ80 transducing phage carrying the ara region, the lac region; F TS, F factor with mutation rendering its replication temperature sensitive; φ80 h, φ80 host range; φ80 v, φ80 virulent; λ hφ80, λ—φ80 hybrid having λ immunity and φ80 host range and attachment specificity. episome has been inserted into the Escherichia coli chromosome at the T1 locus near the attachment site for bacteriophage φ80. From a strain carrying such an insertion, a φ80d ara transducing phage was isolated. The techniques used should be applicable for the isolation of specialized transducing phages for many E. coli genes.
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