Abstract
Pre-treatment of paper pulps with xylanases has been shown to decrease the amounts of toxic chlorine dioxide used to bleach pulp. Natural xylanases are unable to tolerate the extremes of pH and temperature during the paper bleaching process and have to be genetically modified to be made more suitable for such industrial conditions. Such modification can be achieved using site-directed or random mutagenesis methods. Random mutagenesis methods are more attractive because detailed information regarding sequence or structure of the enzyme is not required. This chapter outlines how the thermal stability and alkaline stability of a glycosyl hydrolase family 11 cellulase-free xylanase from the fungus Thermomyces lanuginosus were improved using two random mutagenesis methods, error-prone PCR and a DNA shuffling method called the staggered extension process.
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