Abstract
Ultraviolet photodissociation or UVPD is an increasingly popular option for tandem-mass spectrometry experiments. UVPD can be carried out at many wavelengths, and it is important to understand how the results will be impacted by this choice. Here, we explore the utility of 213nm photons for initiating bond-selective fragmentation. It is found that bonds previously determined to be labile at 266nm, including carbon-iodine and sulfur-sulfur bonds, can also be cleaved with high selectivity at 213nm. In addition, many carbon-sulfur bonds that are not subject to direct dissociation at 266nm can be selectively fragmented at 213nm. This capability can be used to site-specifically create alaninyl radicals that direct backbone dissociation at the radical site, creating diagnostic d-ions. Furthermore, the additional carbon-sulfur bond fragmentation capability leads to signature triplets for fragmentation of disulfide bonds. Absorption of amide bonds can enhance dissociation of nearby labile carbon-sulfur bonds and can be used for stochastic backbone fragmentation typical of UVPD experiments at shorter wavelengths. Several potential applications of the bond-selective fragmentation chemistry observed at 213nm are discussed. Graphical Abstract ᅟ.
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