Abstract
Direct "wet" staining of tumour or haematopoietic colonies in agar culture.
Highlights
After considering and testing a variety of stains, we found that the G-250 form of Coomassie Brilliant Blue (CBB) dye, which stains proteins, satisfied the above requirements
FIG. 1.-Low-power photomicrograph of renal-carcinoma colonies in soft agar stained with CBBG250
Times, plates were examined with an inverted microscope and the colonies were counted after staining
Summary
THE RECENT development of in vitro soft-agar clonogenic assays for human tumour stem cells (Hamburger & Salmon, 1977a, b; Hamburger et al, 1978) provides a very useful tool for study of the biology and growth of various forms of cancer. Our group recently described a method for drying intact colony-containing plating layers directly on to slides and rendering them suitable for a variety of routine and special stains which can be used to verify the cell type of origin of the colonies (Salmon & Buick 1979). After considering and testing a variety of stains, we found that the G-250 form of Coomassie Brilliant Blue (CBB) dye, which stains proteins, satisfied the above requirements.
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