Abstract
OBJECTIVE: ICSI has a definite weak point, which is that it is not possible to visualize the Metaphase-II (M-II) chromosomes during the procedure. A polarized light microscope (POL) offers a clear view of the meiotic spindles but it is difficult to micromanipulate the oocytes under the POL microscope. We report the first successful visualization of human M-II chromosome under an inverted microscope without any special devices or stain. DESIGN: Novel trial for direct visualization of Metaphase-II chromosomes in human oocytes. MATERIALS AND METHODS: The 41 freshly ovulated oocytes were donated by 36 patients. A total of 77 immature oocytes were cultured in-vitro and 42 (54.5%) extruded a polar body. The M-II chromosomes were easily recognized as a round transparent substance in which the chromosome body was centrally located. A total of 90.2% (37/41) [73.0% (27/37) in group A and 27.0% (10/37) in group B] of in-vivo matured and 95.2% (40/42)[62.5% (25/40) in group A and 37.5% (15/40) in group B] of in-vitro matured oocytes had visible chromosomes. Six oocytes in which chromosomes could not be identified were scatters. RESULTS: The 41 freshly ovulated oocytes were donated by 36 patients. A total of 77 immature oocytes were cultured in-vitro and 42 (54.5%) extruded a polar body. The M-II chromosomes were easily recognized as a round transparent substance in which the chromosome body was centrally located. A total of 90.2% (37/41) [73.0% (27/37)] in group A and [27.0% (10/37)] in group B of in-vivo matured and 95.2% (40/42)[62.5% (25/40)] in group A and [37.5% (15/40)] in group B of in-vitro matured oocytes had visible chromosomes. Six oocytes in which chromosomes could not be identified were scatters. CONCLUSION: This new technique will improve embryonic development and help generate better clinical outcome. Furthermore, the visualization of the M-II chromosomes will contribute to the success of nuclear transfer, which is promising for the rescue of low-quality aged oocytes and treatment of mitochondrial diseases.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
