Abstract
3D single molecule localization microscopy (SMLM) has emerged as a powerful method for structural cell biology, as it allows probing precise positions of proteins in cellular structures at a resolution of tens of nanometers in both 2D and 3D. Popular approaches to 3D imaging include PSF engineering to encode the position of single-emitters in its shape. However, such methods lead to worse localization precision axially than laterally. Complex localization microscopy techniques, such as iPALM, can provide isotropic resolution at the cost of a complex instrument.
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