Abstract
A protocol for induction of direct somatic embryogenesis and subsequent plant regeneration for the medicinally important and endangered plant of Malaxis densiflora has been developed for the first time. In the present study, in vitro seed derived protocorm explants were cultured on half strength Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D), Picloram and Dicamba individually and in combination with cytokinins BAP, TDZ and Kn for its effectiveness to induce the differentiation of somatic embryos. The best response was observed in protocorms cultured half strength MS medium supplemented with 2,4-D at 3.39 μM and TDZ at 6.80 μM. Both epidermal and sub epidermal cells were involved in the formation of embryos. The proembryos developed into globular stage and subsequently developed into protocoms. Complete plantlets were formed after 60 days of culture. The plantlets were acclimatized in plastic pots containing sterilized vermiculite. The survival rate was 76%.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.