Abstract
From one week through 7 weeks after artificial pollination, immature ovules of yooza(Citrus junos Sieb. et Tanaka) were excised and cultured in vitro on MT media. Even though there was only a little difference in percentage of somatic embryo formation depending upon the time of excision, immature ovules of 4-week-old showed the highest ratio of somatic embryo formation without callus outgrowth. Various growth regulators or other stimulators were added to the MT media to increase the somatic embryogenesis, In general, BAP was more effective than 2,4-D for somatic embryo formation and the combinations of 0.01mg/l 2,4-D and 0,01 or 0.1mg/l BAP were particularly effective in stimulating somatic embryo formation. When 500mg/l malt extract was added to the medium, the percentage of somatic embryo formation increased reaching as high as 86.7%. Plant regeneration from somatic embryos reached to 66.7% on the medium containing 1.0mg/l zeatin. Isozyme banding patterns were also analyzed to confirm the variations of characteristics of the plantlets derived from direct somatic embryos.
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