Direct shoot organogenesis from different explants of chrysanthemum, marigold, and tuberose

Abstract

Regeneration protocols for direct differentiation of shoots have been developed from different explants of some of the commercially important cultivars of Chrysanthemum morifolium Ramat., F1 hybrid of Tagetes erecta L., and Polianthes tuberosa L. Leaf explants of chrysanthemum differentiated shoot buds in the presence of 6-benzylaminopurine (BA) and indole-3-acetic acid (IAA), while the flower head and receptacle differentiated in the presence of BA and a-naphthaleneacetic acid (NAA). Triiodobenzoic acid played a very crucial role for induction of shoots in leaf explant of F1 hybrid of marigold. Thidiazurone along with NAA induced shoots in the ray florets of marigold. In tuberose, nodular structures were formed in the presence of higher concentrations of BA + gibberellic acid and AgNO3, but the further development of shoot buds from those nodules was possible only when these concentrations were lowered in the presence of abscisic acid and IAA. The plantlets regenerated from different explants were transf...