Abstract
Plant regeneration from cultured immature inflorescence of Curcuma longa was obtained by direct shoot development on Murashige and Skoog's basal medium supplemented with BA (5 or 10 mg l−1) in combination with 2,4-D (0.2 mg l−1) or NAA (0.1 mg l−1) and TDZ (1 or 2 mg l−1) in combination with IAA (0.1 mg l−1). Regenerated shoots were grown on MS medium for further development and later transferred to medium supplemented with 0.1 mg l−1 NAA for induction of roots. Complete plants thus obtained were transferred to sterilized soil in paper cups for 3–4 weeks and then to field, where 95% of the plants survived to maturity.
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