Direct regeneration of shoots from immature inflorescence cultures of turmeric

Abstract

Plant regeneration from cultured immature inflorescence of Curcuma longa was obtained by direct shoot development on Murashige and Skoog's basal medium supplemented with BA (5 or 10 mg l−1) in combination with 2,4-D (0.2 mg l−1) or NAA (0.1 mg l−1) and TDZ (1 or 2 mg l−1) in combination with IAA (0.1 mg l−1). Regenerated shoots were grown on MS medium for further development and later transferred to medium supplemented with 0.1 mg l−1 NAA for induction of roots. Complete plants thus obtained were transferred to sterilized soil in paper cups for 3–4 weeks and then to field, where 95% of the plants survived to maturity.