Direct printing of living cell composed 3D objects with complex and large shape: a scaffold-free approach

Abstract

Event Abstract Back to Event Direct printing of living cell composed 3D objects with complex and large shape: a scaffold-free approach Léa Pourchet1, Christophe Marquette1 and Amélie Thépot2 1 université Lyon 1, ICBMS, France 2 LabSkin Creations, France In the past few years, the advances in 3D printing and especially in bioprinting, gave an excellent overview of what can be the future of the regenerative medicine. Scaffolds for tissues growing and other biomaterial applications were then largely covered by the developed technologies. Nevertheless, instruments, biomaterial formulation and technologies being able to print directly living cells in real 3D format with hollow structures and multi-component composition are still very few. In our present approach, we had created an open source bioprinter on the basis of a 3D extrusion printer (TOBECA®, France), by substituting the extruders’ head with a rack of syringe pumps, enabling the bio-extrusion process. All parts of the new bioprinter were then printed using the initial PLA 3D printer, following the self-replication concept. All source code and design files are now open source available. Ink formulation optimization was carried on in order to permit living cell extrusion. An alginate based ink was selected, together with the additives necessary to the stabilization of the ink and the cell proliferation. The developed ink has a perfect biocompatibility, printability, and an ideal polymerization process adapted to the cell culture. We were thus able to print bi-component 3D hydrogels with high potential applications in the field of regenerative medicine and 3D organ model systems. We have demonstrated the feasibility of printing functional tissue. Indeed, once the objet printed and after few weeks of incubation, we observed the development of cells 3D network as well as the formation of neo-synthesized extracellular matrix. The tissue characterization shows the formation of a dermis and epidermis. These amazing results allow seeing how could be the future of the personalized medicine. The latest developments of the technique and the results of the last living printed 3D objects will be presented and discussed in the light of the biomaterial and tissue engineering community requirements. Keywords: Hydrogel, Bioprinting, complex tissue orgnization Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Three-dimensional fabrication Citation: Pourchet L, Marquette C and Thépot A (2016). Direct printing of living cell composed 3D objects with complex and large shape: a scaffold-free approach. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.01181 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Léa Pourchet Christophe Marquette Amélie Thépot Google Léa Pourchet Christophe Marquette Amélie Thépot Google Scholar Léa Pourchet Christophe Marquette Amélie Thépot PubMed Léa Pourchet Christophe Marquette Amélie Thépot Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.