Direct PCR by the AmpF lSTR NGM™ kit for database purpose

Abstract

To fully maximize the utility of DNA databases for tracing the origins of criminal activity, the streamlining of database sample processing must also be accompanied by high performance chemistries. Blood and buccal samples are often used for convicted-felon DNA profile databases, as these samples are easy to obtain. The AmpF lSTR NGM™ (Next Generation Multiplex) kit provides enhanced performance on degraded and inhibited samples and tolerance to PCR inhibitors, so we tested the kit ability to amplify directly blood and buccal samples deposited on special collecting cards. The ability to amplify stored DNA directly would allow the lab to get results more efficiently by eliminating the DNA purification step, minimizing the possibility of contamination reducing costs and time accelerating testing by as much as 30% so that lab faster would send consistent results to national database.