Direct PCR-Based Detection of Y Chromosome Strs Loci from Human Hair Samples

Hassab El- Nabi E,Geba M,Elroby M

doi:10.21608/eajbsc.2018.22664

Abstract

Direct-PCR is not common for Y chromosome in forensic laboratories in Egypt. The current investigation was carried out to evaluate the priority of direct PCR technique in Y-STR test over routine extraction method.Results showed that direct PCR over hair samples obtained from five persons who do not use any Hair waxes recorded higher scale of peak heights than those subjected to a prior-PCR DNA extraction. In addition, much better results for direct PCR were obtained when one hair was subjected to direct PCR amplification than using three hairs. This technique may solve the problems of rapid and cost-reducing genotyping of forensic samples, as well as may improve the fluorescence detection of some genetic loci.

Highlights

DNA profiling and fingerprint analysis both have considerable distinctive potential that are utilized as a mean of human identification and as an integral part of a forensic laboratory’s workflow (Templeton et al, 2017)
Results presented in table (1) and illustrated in figures (1- 4) showing the data of Y chromosome profiles of both one and three hairs samples for five males who exposed to two technical methods; routine extracted method prior to entering samples to polymerase chain reaction (PCR), and Direct PCR technique
Lengths of the peaks are a strong indicator of the direct PCR method dexterity on the routine extraction method: One hair samples that exposed to direct PCR technique showed preference in the scale of peak heights of most loci than the same samples those were extracted prior to exposure to the PCR, as shown in figure (1)

Summary

Introduction

DNA profiling and fingerprint analysis both have considerable distinctive potential that are utilized as a mean of human identification and as an integral part of a forensic laboratory’s workflow (Templeton et al, 2017). DNA extraction is the first step in forensic analyses in order to obtain adequate amounts of DNA (Sambrook and Russell, 2001). DNA extraction can result in an approximate loss of 20% to 90% of the initial template amount, dependent upon the method of extraction and accuracy of the quantification method (Van Oorschot et al, 2003; Balogh et al, 2003; and Ottens et al, 2013b). The evolved DNA is subjected to PCR and electrophoresis. This process causes a significant loss in sample DNA ranged from 2070% (Van Oorschot et al, 2003) and has the potential to introduce extraneous DNA

Methods

Results

Conclusion