Abstract
A high efficiency plant regeneration protocol has been developed for Punica granatum L. cv. Kandhari Kabuli using hypocotyl explants. Hypocotyl explants (about 0.5–1.0 cm in size) were procured from 15 to 20-day-old in vitro germinated seedlings. The highest frequency of shoot regeneration (68.21 %) along with 3.18 average number of shoots per explant was obtained via direct organogenesis from hypocotyl explants cultured on Murashige and Skoog’s medium supplemented with 2.0 mg/l 6-benzyl amino purine and 1.5 mg/l α-naphthalene acetic acid. High percentage root regeneration (85.62 %) in in vitro developed shoots was obtained on half strength Murashige and Skoog’s medium supplemented with 0.10 mg/l α-naphthalene acetic acid and 0.02 % activated charcoal. Pomegranate plantlets were able to regenerate within 6 weeks and well rooted plantlets were successfully acclimatized in plastic cups containing autoclaved peat moss. They were weaned in a growth room and finally moved to a greenhouse. This is the first report on direct organogenesis in P. granatum L. cv. Kandhari Kabuli using hypocotyl explants. This regeneration protocol will be very useful for undertaking genetic manipulation providing a value addition to pomegranate propagation in future.
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Schedule a callMore From: Proceedings of the National Academy of Sciences, India Section B: Biological Sciences
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