Abstract
Cotyledonary node explants from 3 - 5-d-old seedling derived explants of Cucurbita pepo (L.) produced multiple shoots in Murashige and Skoog (MS) salts B5 vitamins containing medium in the presence of N6-benzylamino-purine BAP 1 mg/L + Thidiazuron TDZ 0.05 mg/L. After 4 weeks explants were subcultured to medium containing MS salts B5 Vitamins BAP 0.5 mg/L, Gibberellic acid GA3 1 mg/L and L-Glutamine 15 mg/L. Periodic excision of regenerated shoots from the explants increased shoot efficiency during subculture. The combination of L-Glutamine with BA and GA3 significantly increased the shoot proliferation. Elongated shoots were excised from shoot clumps and transferred to rooting medium containing indole-3-butyric acid (IBA, 1.0 mg/L). The rooted plants were hardened in small pot containing standardized garden soil, well developed plant transferred to greenhouse and survival rate was 85%.
Highlights
The Cucurbita genus, of American origin, is one of the most variable genera within the Cucurbitaceae family [1]
The objective of this study was to investigate the effect of age of the explant, effect of important additives and subcultures to improve the regeneration from cotyledonary node explants of important zucchini type vegetable crop
Adventitious buds were induced from the meristematic regions of cotyledonary node explants on Murashige and Skoog (MS) medium supplemented with BA (0.5 - 2 mg/L) or KN (0.5 - 2 mg/L) or TDZ (0.01 - 0.2 mg/L) after 2 weeks of culture
Summary
The Cucurbita genus, of American origin, is one of the most variable genera within the Cucurbitaceae family [1]. Summer squashes of the Zucchini type rank among the highest-valued vegetables worldwide, whereas the “winter squash” types (fruits consumed when mature) of Cucurbita pepo and related Cucurbita spp. are food staples and rich sources of fat and vitamins in developing countries [2]. It has various medicinal effects comprising antidiabetic, antihypertensive, antitumor, antimutagenic, immunomodulating, antibacterial, anti-hypercholesterolemic, intestinal antiparasitic, antalgic, and anti inflammation effects, and utilization possibilities of various Cucurbitaceae species have been reported [3].
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