Direct Organogenesis and Plantlet Regeneration from Mature Zygotic Embryos of Chinese Pine(Pinus tabulaeformis Carr.)

Abstract

Mature zygotic embryos of Chinese pine were cultured as initial explants to investigate the process of direct organogenesis.Adventitious buds were initiated on DCR and MS medium supplemented with 1~5 mg·L-1 benzyladenine(BA) with or without 1~10 mg·L-1 2,4-dichlorophenoxyacetic acid(2,4-D).The highest induction frequency of adventitious buds(90%) was obtained on DCR medium containing 1 mg·L-1 BA.Subsequent transfer of buds to plant growth regulator(PGR)-free medium in time was advantageous for elongation of adventitious buds.1/2DCR medium with 1 mg·L-1 indole-3-butyric acid(IBA) was suitable for adventitious root initiation,on which average rooting frequency was 47.1%.Plantlets were successfully acclimatized in vermiculite and transferred to greenhouse,where they had a survival rate of 86.4% and no morphological abnormalities.