Abstract
Flash-induced absorption changes in the UV have been monitored with nanosecond time resolution in oxygen-evolving photosystem II complexes from Synechococcus sp. At 260 nm, the oxidation of the immediate donor to Chl- a + II (P-680 +) in the 10–500 ns range could be observed directly. The difference extinction coefficient for its oxidation is estimated to be 6000–8000 M −1·cm −1 at 260 nm. The observed kinetics for the oxidation of the donor is identical with the reduction kinetics of Chl- a + II as monitored at 824 nm. At 320 nm, the reduction of Q A was found to be faster than 1 ns.
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