Abstract
Type-II DNA topoisomerases(topoII) are ubiquitous enzymes that play key roles in the maintenance of DNA topology in cells. They control the degree of supercoiling of DNA and untangle the catenanes that arise during replication or recombination. Lack of their activities during cell division ultimately causes cell death. TopoII untangles DNA catenanes in an ATP-dependent manner, by catalyzing the transport of one DNA segment to the other side of a second DNA segment through a transient double-stranded break in the second segment. The work of topoII would seem like that of a magician who fascinates the audience, by solving a knot of rope without touching the knot.Here, we show movies of this unlinking magic taken in real time under an optical microscope.
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