Direct Observation of Adhesion and Mechanical Behavior of a Single Poly(lactic-co-glycolic acid) (PLGA) Fiber Using an In Situ Technique for Tissue Engineering.

Abstract

Nanometer- and submicrometer-sized fiber have been used as scaffolds for tissue engineering, because of their fundamental load-bearing properties in synergy with mechano-transduction. This study investigates a single biodegradable poly(lactic-co-glycolic acid) (PLGA) fiber's load-displacement behavior utilizing the nanoindentation technique coupled with a high-resolution in situ imaging system. It is demonstrated that a maximum force of ∼3 μN in the radial direction and displacement of at least 150% of fiber diameter should be applied to acquire the fiber's macroscopic mechanical properties for tissue engineering. The adhesion behavior of a single fiber is captured using a high-resolution camera. The digital image correlation (DIC) technique is adopted to quantify the adhesion force (∼25 μN) between the fiber and the tip. Adhesion force has also been quantified for the fiber after immersing in phosphate-buffered saline (PBS) to mimic the bioenvironment. A 4-fold increase in adhesion force after PBS treatment was observed due to water penetration and hydrolysis on the fiber's surface. A high similarity between mechanical properties of a single fiber and native tissues (elastic modulus of 10-25 kPa) and superior adhesion force (25-107.25 μN) was observed, which is excellent for promoting cell-matrix communication. Overall, this study examines the mechanics of a single fiber using innovative indentation and imaging processing techniques, disclosing its profound and striking roles in tissue engineering.