Abstract
Applications of 13C nmr in following the metabolism and fate of 13C enriched substrates in whole cells are described. Studies on the elaboration of coproporphyrinogens I (2a) and III (2b) from [5-13C]-δ-aminolevulinic acid (1) and [11-13C-porphobilinogen (PBG, 3), in Rhodopseudomonasspheroides and Propionibacteriumshermanii, respectively, the randomization of 13C from [3-13C]-propionate by P. shermanii, the biosynthesis of citrate, gentisalcohol (4), and patulin (5) from [2-13C]-acetate by Penicilliumurticae, and the anaerobic metabolism of [1-13C]-glucose in rabbit erythrocytes are reported.
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