Abstract

Samples of Nile and Ismailia canal water were subjected to direct inoculation and incubation at elevated temperatures. The results obtained showed that the use of MacConkey broth medium was better than EC broth. In addition, the Direct MPN technique revealed to be suitable and superior by 90% of the examined samples. A total of 300 isolates was recovered from Direct MPN-technique and identified by using IMViC reactions plus Eijkman test. 96% of the isolates were E. coli proper and 4% irregular types.

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