Abstract
UvrD is a prototypical Superfamily 1 (SF1) helicase from E. coli involved in DNA repair. We report herein the directmeasurement of the stepping behavior of UvrD at thesingle-molecule level as it unwinds and re-zips a DNA hairpin usinghigh-resolution optical tweezers. We measure an averageunwinding and re-zipping step size of ∼3 base pairs, but also observe large variation in step size.Analysis of the step size distribution reveals the presence of multiple sub-steps smaller than 3 bp,suggesting that 3 bp is not the elemental unit of step sizefor UvrD.
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