Abstract
Four narrow lines are observed in the high-resolution cross-polarization magic-angle spinning 13C NMR spectra of intact, lyophilized samples of Pseudomonas sp. LBr, in addition to the broader lines normally associated with bacterial cellular material. These narrow lines arise from poly(beta-hydroxybutyrate). The cellular carbon contained in this storage material can be measured quantitatively and nondestructively from the solid-state NMR spectra. We find that cells starved for phosphorus store up to 50% of their total carbon as poly(beta-hydroxybutyrate). When such cells are used to inoculate medium containing a source of phosphorus, all of the poly(beta-hydroxybutyrate) is metabolized by the time the culture has reached midlogarithmic growth phase.
Highlights
From the Momanto Company, PhysicalSciences Center, St
Four narrow lines are observed in the high-resolution cross-polarization magic-angle spinning I3C NMR spectra of intact, lyophilized samples of Pseudomonas sp
We find that cells starvedfor phosphorus store up to 50%of their total carbon as poly(8-hydroxybutyrate).When such cells are used to inoculate medium containing a source of phosphorus, MATERIALS AND METHODS
Summary
From the Momanto Company, PhysicalSciences Center, St. Louis, Missouri 63167 signalsfrom 13C or 15N nuclei and magic-angle spinning (MAS) with proton dipolardecoupling for high resolution. Four narrow lines are observed in the high-resolution cross-polarization magic-angle spinning I3C NMR spectra of intact, lyophilized samples of Pseudomonas sp. LBr, in addition to the broader lines normally associated with bacterial cellular material. NMR spectra of lyophilized cells of a PHB-producing bacterium contain narrow lines attributable to PHB. The intensities of these lines can be used to monitor direcstilmy,ply, and nondestructively the PHB produced in culture under varying growth conditions. We find that cells starvedfor phosphorus store up to 50%of their total carbon as poly(8-hydroxybutyrate).When such cells are used to inoculate medium containing a source of phosphorus, MATERIALS AND METHODS
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
