Abstract
NO production in platelets has been followed by electrochemical detection. It was undetectable in unstimulated platelets and in thrombin or ADP-stimulated platelets, but dose-dependently stimulated by collagen. A production of 5 10 −19 mol/platelet was reached with 9 μg collagen. In collagen-stimulated platelets, preincubation with 1 mM L-Arg, D-Arg or L-NMMA increased by 77%, left unchanged or decreased by 63% NO production, respectively. NO production did not parallel cytosolic Ca 2+ changes, although it decreased in low Ca 2+ medium or when Ca 2+ transients were attenuated by intracellular Ca 2+ buffer. These results confirm that human platelets can generate NO. They demonstrate that cytosolic [Ca 2+], although participating in the regulation of its synthesis, is not the messenger for NO synthase activation. Platelet NO production could become functionally important when collagen fibrils of the sub-endothelium are accessible.
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