Abstract
A sensitive electrochemical method for the direct determination of cholesterol is described. Cholesterol adsorded on a hanging mercury drop electrode can be oxidized during a linear potential scan. The electrode reaction is reversible in 0.1 M KCl solution with oxidation and reduction peak potentials at −0.05 and −0.075 V, respectively. The adsorption of cholesterol is used as an accumulation step, and then the surface are measured by stripping voltammetry. The adsorptive stripping response was evaluated with respect to stripping mode, electrolyte solution, accumulation time, concentration dependence, possible interferences and other variables. The peak current was linear with cholesterol concentration in the rate of 0.1–8 μM. The detection limit was 0.06 μM after 3 min accumulation. The relative standard deviation at 6 μM was 5.5%. Application to the determination in human serum was assessed.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
