Direct injection mass spectrometry reveals unique metabolite profiles from spent human embryo culture media due to albumin source and pregnancy outcome

Abstract

Identification of biomarkers associated with embryo viability will facilitate the move to single embryo transfer. Here we employed a rapid, high-throughput direct injection-mass spectrometry (DI-MS) technology to analyze spent human embryo culture media samples to resolve a unique metabolic signature related to pregnancy outcome, and media supplemented with either human serum albumin (HSA), or a high/low dose of recombinant human serum albumin (rHSA).