Abstract
The present study established a direct immunodiffusion precipitin method for the determination of immunoglobulin levels. Agar gel was solidified on a glass slide and the desired number of wells were made. Five μl of test samples were placed in each well of agar gel and the serum proteins were allowed to diffuse for a period of adequate time. An aliquot size of Whatman No.1 filter paper which has been impregnated with antiserum was placed on the agar gel and kept in a moist chamber for 1 to 3 h depending on the immune reaction. The precipitin ring was clearly observed in an agar plate and the diameter of the ring could be measured before and after staining. The present method is economical, using a small quantity of antiserum when compared with the previously described methods and the results can be obtained rapidly with a sharply edged ring.
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