Abstract
Given the profusion of biotechnological applications of the nonaqueous use of lipases, we have evaluated the possibilities of exploiting the inherent advantages of high performance liquid chromatography (HPLC) for a simple, rapid assay of lipase activity in reverse micellar media, as a convenient alternative to previously reported spectroscopic methods, using both a model system and esterification reaction, and different commercial lipases. The results obtained after a screening for optimized chromatographic conditions in the reverse-phase mode indicate that a satisfactory resolution of the reaction components can be obtained following a straightforward protocol, which permits an accurate, reliable quantitation of the reaction progress, regardless of the enzyme used.
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