Abstract
A rapid assay has been developed for on-line SPE-HPLC determination of trimethoprim in milk. SPE precolumns packed with reversed-phase sorbent (RPS C-18) and with restricted access material (ADS C-18) have been tested in the column switching system. Only a simple preparation procedure for the milk sample is required before the direct injection. A Symmetry C-18 analytical column and mobile phase consisting of 100 mM phosphate buffer (pH3), with the addition of an ion-pair agent (PSA) and acetonitrile, have been used for HPLC analysis. UV detection was performed at 229 nm. The detection limit for trimethoprim in milk was 5 ng/mL.
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