Abstract
AbstractA methodology for microarray based epigenomic profiling is presented. The method relies on platinum-based fluorescence labeling reagents for direct (non-enzymatic) labeling of DNA and RNA. This is a work in progress and only preliminary data is presented here.
Highlights
Platinum-based reagents (such as ULS from Kreatech) selectively react with and label the N7 of Guanine residues slide 2/7
Platinum-based reagents selectively react with and label the N7 of Guanine residues slide 2/7
Genomic DNA can be differentially digested with methylation-sensitive restriction enzymes and directly fluorescently labeled slide 4/7
Summary
Platinum-based reagents (such as ULS from Kreatech) selectively react with and label the N7 of Guanine residues slide 2/7. Direct fluorophore conjugation to genomic DNA for microarray-based epigenomic profiling Non-Enzymatic Conjugation of Fluorescent Dyes to Nucleic Acids Conjugated mRNA Produces Highly Precise Expression Profiles
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