Direct evidence of the important role of proteins in bioconcentration and biomagnification of PFASs in benthic organisms based on comparison with OPEs

Abstract

Despite the wide acceptance that bioconcentration and biomagnification of per/polyfluoroalkyl substances (PFASs) is related to proteins in organisms, few direct evidences are available. Here, bioconcentration and biomagnification of 9 organophosphate esters (OPEs) and 16 PFASs, which have similar range of log Kow (octanol-water partitioning coefficient) values, were compared in the benthic food chain of biofilm-snail in Taihu Lake, China. The ∑OPEs level in water (150–23,036 ng/L) was significantly higher than ∑PFASs (57.3–351 ng/L). Although the logarithm of bioconcentration factors of both OPEs and PFASs in biofilm positively correlated with their log Kow, the slope of PFASs was 4 times of that of OPEs, which might be due to the strong interactions of PFASs with biofilm extracellular proteins. Additionally, PFASs exhibited distinctly greater biomagnification factors from biofilm to snails (3.09–17.8) than OPEs (0.39–3.48). Significant correlations between the concentrations and protein contents in snails were observed for most long-chain PFASs, but not for any OPEs. Multiple receptor models identified polyurethane foam (77.9 %) and food packaging/metal plating (56.9 %) were the primary sources of OPEs and PFASs in Taihu Lake, respectively. We provided strong and direct evidences that proteins facilitated bioconcentration and biomagnification of PFASs.