Abstract
(Biophysical Journal 115, 313–327; July 17, 2018) In Eq. 4, the average electron density of glycerol was incorrectly published as 0413. The correct value is 0.413. The correct equation appears below and has been corrected online.Dshell=0.413βVf+0.367(1−βVf) Direct Evidence for the Effect of Glycerol on Protein Hydration and Thermal Structural TransitionHirai et al.Biophysical JournalJuly 17, 2018In BriefThe mechanisms of protein stabilization by uncharged solutes, such as polyols and sugars, have been intensively studied with respect to the chemical thermodynamics of molecular crowding. In particular, many experimental and theoretical studies have been conducted to explain the mechanism of the protective action on protein structures by glycerol through the relationship between hydration and glycerol solvation on protein surfaces. We used wide-angle x-ray scattering (WAXS), small-angle neutron scattering, and theoretical scattering function simulation to quantitatively characterize the hydration and/or solvation shell of myoglobin in aqueous solutions of up to 75% v/v glycerol. Full-Text PDF Open Archive
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