Abstract
Insertional inactivation of the trpE gene in Corynebacterium glutamicum AS019 blocked expression of the trp operon from the primary promoter and resulted in tryptophan auxotrophy. The presence of indole, the substrate for the terminal trp pathway enzyme tryptophan synthase, reversed this auxotrophy thereby providing evidence for the existence of an internal promoter independently expressing the tryptophan synthase genes (trpB and trpA). The toxic tryptophan analogue 6-fluorotryptophan failed to inhibit the growth permitted by indole thus suggesting that expression of tryptophan synthase from the internal promoter is constitutive.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.