Abstract
The direct and rapid chiral analysis of methorphan was achieved with excellent sensitivity and selectivity using ultra high-performance liquid chromatography-single quadrupole mass spectrometry (HPLC-SQD) and supercritical fluid chromatography-single quadrupole mass spectrometry (SFC-SQD). The optimized separation of dextromethorphan and levomethorphan by HPLC-SQD was accomplished in under 9min using a Chirobiotic V2 (2.1×250mm, 5μm) column with a mobile phase consisting of MeOH/NH4OH/HOAc (100/0.02/0.1, v/v/v). The optimal separation by SFC-SQD was achieved in less than 3min using a Trefoil AMY1 (2.1×150mm, 2.5μm) column with a mobile phase comprised of CO2, 9% MeOH as a co-solvent, and 0.3% cyclohexylamine as a modifier. The limit of detection was 10ng/mL in the selected ion recording mode for both systems.
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