Abstract
Cytochrome c (Cyt c) was successfully immobilized on poly(ferrocenylsilane)–DNA (PFS–DNA) composite film modified gold electrode by electrostatic adsorption. The direct electron transfer of Cyt c in the composite film modified electrode and its application as hydrogen peroxide (H 2O 2) biosensor were investigated. The results suggested that Cyt c could be effectively immobilized in the PFS–DNA composite modified electrode. The adsorbed Cyt c showed a good electrochemical activity with a pair of quasi-reversible redox peaks in 0.20 M, pH 7.0 phosphate buffer solution. PFS–DNA composite films showed an obvious promotion for the direct electron transfer between Cyt c and the underlying electrode. The immobilized Cyt c also exhibited a good electro-catalytic activity towards the reduction of H 2O 2. The catalytic current increased linearly to the H 2O 2 concentration in a range of 3.0 μM–1.83 mM ( r = 0.999; n = 19) and the detection limit was calculated to be 0.72 μM based on the criterion of a signal-to-noise ratio of 3. Based on the novel construction, a third-generation biosensor could be obtained for the determination of H 2O 2.
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