Abstract
Rod-constructed zinc oxide (ZnO) microspheres (RZnOMs), consisting of hundreds of needle-like ZnO nanorods, were utilized to explore a novel biosensor through coupling with myoglobin (Mb) in the presence of chitosan (Chi). Biocompatibility and electrochemical properties of the resulting ZnO-Chi-Mb composite film were studied by Fourier-transform infrared spectroscopy and cyclic voltammetry. The results revealed that the RZnOMs-based composite was a satisfying matrix for proteins to effectively retain their native structure and bioactivity. With advantages of the unique inorganic material, facilitated direct electron transfer of the metalloenzymes was acquired on the RZnOMs-based enzyme electrode. Moreover, the RZnOMs-based biosensor also displayed significant electrocatalytic activity for the reduction of hydrogen peroxide with an apparent Michaelis–Menten constant (32 µM), wide linear range (2–490 µM), and low detection limit (0.21 µM, S/N = 3). These indicated that the RZnOMs were one of the ideal candidate materials for direct electrochemistry of redox proteins and related biosensor construction.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
