Abstract

Direct electrochemistry of hemoglobin was observed in stable thin film composed of a natural lipid (egg–phosphatidylcholine) and hemoglobin on pyrolytic graphite (PG) electrode. Hemoglobin in lipid films shows thin layer electrochemistry behavior. The formal potential E°′ of hemoglobin in the lipid film was linearly varied with pH in the range from 3.5 to 7.0 with a slope of −46.4 mV pH −1. Hemoglobin in the lipid film exhibited elegant catalytic activity for electrochemical reduction of H 2O 2, based which a unmediated biosensor for H 2O 2 was developed.

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