Direct electrochemistry of cytochrome c on a graphene/poly (3,4-ethylenedioxythiophene) nanocomposite modified electrode

Abstract

The direct electrochemistry of cytochrome c (Cyt c) embedded in a novel support matrix of graphene/poly (3,4-ethylenedioxythiophene) (G-PEDOT) nanocomposite on a glassy carbon electrode was studied. The prepared G-PEDOT nanocomposite is of good biocompatibility, enhanced electric conductivity and large specific surface area. The immobilized Cyt c in G-PEDOT matrix (graphene weight percentage<2%) displays excellent direct electrochemistry and retains its biocatalytic activity toward the reduction of hydrogen peroxide. The electrochemical signal shows a linear response to H2O2 in the concentration range from 5.0×10−7 to 4.0×10−4M with a detection limit of 2.49×10−7M. The results demonstrate that the G-PEDOT nanocomposite offers a biocompatible material for the construction of biofuel cells, bioelectronics and biosensors.