Abstract
The direct electrochemistry of fresh tobacco RNA on the surface of the pretreated glassy carbon electrode (PGCE) is researched. Tobacco RNA immobilized at PGCE shows good electrochemical activity in 0.10 M pH 7.5 phosphate buffer solution. The typical surface-controlled & irreversible electrode process of tobacco RNA was achieved by cyclic voltammetry and differential pulse voltammetry with the participation of two electrons and two protons, providing a paradigm for the direct electrochemical study of RNA. In addition, cyclic voltammetry and amperometry techniques are employed to demonstrate that tobacco RNA displays the characteristics of peroxidase and exhibits excellent electrocatalytic oxidation to H2O2. The electrochemical H2O2 sensor based on the RNA exhibits a good response, which shows a range of 5.0 × 10−6–2.5 × 10−4 M with a low detection limit of 1.7 × 10−6 M (S/N = 3).
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