Abstract
In this paper three-dimensional graphene (3D-GR) was directly formed on the surface of carbon ionic liquid electrode (CILE) by electrodeposition. By using 3D-GR/CILE as the substrate electrode, a new electrochemical biosensor was prepared by immobilization of hemoglobin (Hb) on the electrode surface with a chitosan film. Electrochemical investigation indicated that a pair of well-defined redox peaks appeared on cyclic voltammogram, indicating the realization of direct electron transfer of Hb with the underlying electrode. The result can be ascribed to the porous structure of 3D-GR with high conductivity and big surface area. Based on the electrochemical data, the electron transfer coefficient (α) and the apparent heterogeneous electron transfer rate constant (ks) were calculated to be 0.426 and 1.864s−1, respectively. The modified electrode displayed good electrocatalytic activity to the reduction of trichloroacetic acid (TCA), and the catalytic reduction peak current had a good linear relationship to TCA concentration in the range from 0.4 to 26.0mmol/L with the detection limit of 0.133mmol/L (3σ). Therefore a new third-generation electrochemical Hb biosensor based on 3D-GR/CILE was constructed with good stability and reproducibility.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
